| Accession number;05A0760589 |
| Title;Asymmetric PCR Using the Primers Anchored on the Surface of Magnetic Nanoparticles |
| Author;SHEN HEBAI(Life And Environment Sci. Coll., Shanghai Normal Univ.) ZHOU HAIQING(Life And Environment Sci. Coll., Shanghai Normal Univ.) WANG YOUBAO(Life And Environment Sci. Coll., Shanghai Normal Univ.) YANG ZHONGNAN(Life And Environment Sci. Coll., Shanghai Normal Univ.) WANG CHEN(Life And Environment Sci. Coll., Shanghai Normal Univ.) |
Journal Title;Bull Chem Soc Jpn
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Journal Code:G0450A
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ISSN:0009-2673
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VOL.78;NO.9;PAGE.1649-1653 (J-STAGE)(2005)
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| Figure&Table&Reference;FIG.8, REF.22 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Asymmetric PCR is generally used to produce ssDNA, which could function as probes for detecting various kinds of genes. An asymmetric PCR technique based on magnetic nanoparticles (MNPs) has been developed in this work. One of the two amplification primers was bound to the MNP surface by modifying its 5'-end and leaving the 3'-end available for DNA polymerase activity, while the other was unbound. The results obtained showed that PCR could proceed successfully when the concentration ratio of the unbound primer to the MNP-bound primer was from 1:100 to 1:10. Plenty of target ssDNA connected with MNPs (MNP-ssDNA complexes) along with some dsDNA with one strand connected to MNPs could be generated. In virtue of their immobilization on the MNP surface, asymmetric PCR products could be easily isolated by the external magnetic field. This new surface asymmetric amplification process can be used not only to produce MNP-ssDNA complexes, but also to provide an alternative route to isolate products of asymmetry PCR. (Author abst.) |
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