Genetic Diversity of Castanopsis cuspidata (Thunb.) Schottky var. sieboldii (Makino) Nakai Distributed in Ryukyu Islands, Japan

Accession number;05A0364410
Title;Genetic Diversity of Castanopsis cuspidata (Thunb.) Schottky var. sieboldii (Makino) Nakai Distributed in Ryukyu Islands, Japan
Author;VIDHANAARACHCHI VIJITHA R. M.(Univ. Ryukyus, Okinawa, Jpn)   BABA SHIGEYUKI(Univ. Ryukyus, Okinawa, Jpn)   MURAYAMA SEIICHI(Univ. Ryukyus, Okinawa, Jpn)   
Journal Title;Japanese Journal of Tropical Agriculture
Journal Code:F0874A
ISSN:0021-5260
VOL.49;NO.1;PAGE.53-60(2005)
Figure&Table&Reference;FIG.2, TBL.5, REF.24
Pub. Country;Japan
Language;English
Abstract;Castanopsis cuspidata var. sieboldii predominates in most islands of the Ryukyu Islands, Japan, consisting of 3 main groups of islands, the Okinawa Islands, the Miyako Islands and the Yaeyama Islands. Although C. cuspidata var. sieboldii is one of the most economically important trees, little information is available about its genetic diversity. Genetic diversity was examined, using 6 microsatellite loci and 216 mature trees from 10 populations distributed in 3 islands, Okinawa, Ishigaki and Iriomote Islands. All the loci were highly variable, and the expected values of heterozygosity (HE) ranged from 0.862 to 0.927. Observed heterozygosity (HO) showed a significant deviation from Hardy-Weinberg expectation at 5 of the 6 loci, and relatively high miss-amplification was observed at 3 loci (12.5%, 11.5% and 7.4%). Since HO of 6 populations showed a significant deviation from Hardy-Weinberg equilibrium with positive FIS values, it was suggested that some level of self-pollination had occurred. Relatively higher levels of homozygotes were prominent in some populations. Most of the genetic variation was observed within the populations, resulting in a lower but significant variation between populations. The deviations may be due to the apparent shortage of heterozygotes caused by self-pollination, miss-amplification of individuals and the presence of null alleles. Genetic distance of the populations within the 2 islands (Iriomote and Ishigaki Islands) of the Yaeyama Islands was relatively small, while the genetic distance between the populations of Yaeyama Islands and Okinawa Island was greater than that within the Yaeyama Islands and Okinawa Island.... (author abst.)