Determination of Oxycodone and Hydrocotarnine in Cancer Patient Serum by High-Performance Liquid Chromatography with Electrochemical Detection
| Accession number;05A0362076 |
| Title;Determination of Oxycodone and Hydrocotarnine in Cancer Patient Serum by High-Performance Liquid Chromatography with Electrochemical Detection |
| Author;KOKUBUN HIDEYA(Kitasato Univ. Hospital, Kanagawa, Jpn) OUKI MAKIKO(St. Marianna Univ. School Of Medicine Hospital, Kanagawa, Jpn) MATOBA MOTOHIRO(Kitasato Univ. School Of Medicine, Kanagawa, Jpn) KUBO HIROAKI(Kitasato Univ., Tokyo, Jpn) HOKA SUMIO(Kitasato Univ. School Of Medicine, Kanagawa, Jpn) YAGO KAZUO(Kitasato Univ. Hospital, Kanagawa, Jpn) |
Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.3;PAGE.337-339(2005)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.3 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotarnine in cancer patients serum. An eluent of methanol:acetonitrile: 5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotarnine were less than 4.8%. The method developed here was better than the method reported previously. (author abst.) |
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