A Protocol for Efficient Development of Microsatellite Markers.
| Accession number;04A0421669 |
| Title;A Protocol for Efficient Development of Microsatellite Markers. |
| Author;LIAN C(Univ. Tokyo, Asian Natural Environmental Sic. Center, JPN) HOGETSU TAIZO(Univ. of Tokyo) |
Journal Title;Journal of the Japanese Forestry Society
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Journal Code:F0858A
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ISSN:0021-485X
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VOL.86;NO.2;PAGE.191-198(2004)
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| Figure&Table&Reference;FIG.4, TBL.3, REF.37 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Because microsatellite (SSR) markers are highly polymorphic, codominant and reproducible, they are widely used as effective genetic markers in many fields such as ecology, breeding and population genetics. One of the major drawbacks of SSR polymorphism analysis is derived from the fact that several species-specific SSR markers must usually be developed for the first time when research is commenced on a species which has never been analyzed before. Especially when the species has SSR loci in the genome at a low frequency, the marker development often requires much labor, time and expense, hindering SSR analysis. Only recently, we have newly established techniques for SSR marker development named dual-suppression-PCR and ISSR-dual-suppression-PCR techniques, and developed much easily and efficiently many SSR markers from many kinds of organisms by these techniques. In this brief review, we described the practical protocol of dual-suppression-PCR technique in detail, for convenience of researchers who challenge SSR marker development. (author abst.) |
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