High-Performance Liquid Chromatographic Assay of Clonazepam in Human Plasma Using a Non-porous Silica Column
| Accession number;04A0432064 |
| Title;High-Performance Liquid Chromatographic Assay of Clonazepam in Human Plasma Using a Non-porous Silica Column |
| Author;NAKAMURA M(Gifu Univ. Hospital, Gifu, Jpn) FUKAWA K(Gifu Univ. Hospital, Gifu, Jpn) SUGIYAMA T(Gifu Univ. Hospital, Gifu, Jpn) KATAGIRI Y(Gifu Univ. Hospital, Gifu, Jpn) |
Journal Title;Biol Pharm Bull
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Journal Code:S0989A
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ISSN:0918-6158
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VOL.27;NO.6;PAGE.893-895(2004)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.14 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A new HPLC method has been developed for measuring clonazepam (CZP) in plasma, using a reversed-phase non-porous silica column packed with 2.MU.m particles. CZP in plasma was first purified with a column extraction technique and injected onto a non-porous silica column. The calibration curve was linear from 5-200 ng/ml. The recoveries of CZP added to plasma were more than 94.0%, with a coefficient of variation in the range of 5.1-13.8%. We developed a rapid routine method using a non-porous silica column that was accurate and improved solvent consumption in the measurement of CZP. (author abst.) |
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