| Accession number;03A0059388 |
| Title;Enhanced Expression of Endothelial Nitric Oxide Synthase and Caveolin-1 in Human Cirrhosis. |
| Author;YOKOMORI HIROAKI(Kitasato Inst.) ODA MASAYA(International Univ. Health and Welfare, JPN) ISHII HIROMASA(Keio Univ.) |
Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.30;NO.Suppl.2;PAGE.S.445-S.447(2002)
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| Figure&Table&Reference;FIG.2, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Background and aims: Nitric oxide is synthesized in diverse mammalian tissues by a family of calmodulin-dependent nitric oxide synthases (NOS). Caveolin, the principle structural protein in caveolae, interacts with endothelial NOS (eNOS) leading to enzyme inhibition. The aim of the present study was to examine the localizations of eNOS and caveolin-1 at protein level in normal human liver tissue, and how the expressions are altered in cirrhotic liver. Methods: Fresh liver specimens were obtained from hepatic surgeries. Normal portions resected from cases of metastatic liver carcinoma were used as control specimens, and cirrhotic portions resected from cases of hepatocellular carcinoma with hepatitis C-related cirrhosis were used as cirrhotic specimens. Anti-eNOS and anti-caveolin-1 antibodies were used for immunohistochemistry. Results: Immunohistochemistry revealed that both eNOS and caveolin-1 were sparsely expressed on hepatic sinusoidal lining in normal liver specimens. Immunohistochemistry demonstrated over-expression of eNOS and caveolin-1 in cirrhotic liver specimens. Conclusion: In cirrhotic human liver, marked increase of caveolin-1 in perisinusoidal cells may promote caveolin-eNOS binding and reduce the activity of eNOS despite leading to impaired NO production and increased hepatic microvascular tone. (author abst.) |
