| Accession number;01A0400199 |
| Title;Inhibitory Mechanism of NaCN on the Smooth Muscle of Guinea Pig Ileum. |
| Author;SHIMIZU KAZUMASA(Nippon Vet. and Anim. Sci. Univ.) ABE TAMAMI(Nippon Vet. and Anim. Sci. Univ.) OZEKI EMI(Nippon Vet. and Anim. Sci. Univ.) ICHIKAWA TAEKO(Nippon Vet. and Anim. Sci. Univ.) URAKAWA NORIMOTO(Nippon Vet. and Anim. Sci. Univ.) NAKAJO SHINJIRO(Nippon Vet. and Anim. Sci. Univ.) |
Journal Title;Bulletin of Nippon Veterinary and Animal Science University
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Journal Code:S0144B
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ISSN:0373-8361
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VOL.;NO.49;PAGE.13-21(2000)
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| Figure&Table&Reference;FIG.8, REF.23 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In guinea pig ileal longitudinal smooth muscle, the high K+ - or Carbachol(CCh)-induced contraction was inhibited by an addition of NaCN in a concentration-dependent manner. NaCN inhibited the increase in oxidized flavoprotein fluorescence by high K+ or CCh, but it enhanced the increase in reduced pyridine nucleotide fluorescence. High K+ or CCh induced a contraction with an increase in intracellular Ca2+ ([Ca2+]i) level. NaCN inhibited the high K+- or CCh-induced contraction by decreasing [Ca2+]i level. When NaCN was applied to the muscle, the rate of O2 consumption was significantly reduced, and decreased O2 consumption was not restored by Ca2+ addition. In summary, by the simultaneous measurement of the fluorescence of PNred and FPox with force development, the inhibitory effect of NaCN on high K+- and CCh-induced contraction of the longitudinal muscle of guinea-pig ileum was confirmed to be the result of the inhibition of aerobic metabolism. NaCN was suspected as not being involved in the inhibition of Ca2+ influx by the ATP-sensitive K+ channel activation which chromakalim displays. (author abst.) |
