| Accession number;00A1007264 |
| Title;Plant Regeneration of Japanese Pea Cultivars by In Vitro Culture of Immature Leaflets. |
| Author;FUJIOKA TADASHI(Wakayama-Ken Norin Suisan Sogo Gijutsu Senta, Nogyo Shikenjo) FUJITA MASAYOSHI(Shinshu Univ., Fac. of Agric.) IWAMOTO KAZUYA(Wakayamakennorinsuisansogise Danchiengeise) |
Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.69;NO.5;PAGE.656-658(2000)
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| Figure&Table&Reference;FIG.1, TBL.2, REF.8 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;To establish a cultural method for regenerating Japanese pea (Pisum sativum L.) cultivars, varietal differences in callus formation, shoot and root regeneration by in vitro culture of immature leaflets were investigated. The effects of the developmental stage of pea explants on these processes were also examined. 1. Shoot regeneration occurred in 5 ot 6 Japanese pea cultivars from primary calli induced from immature leaflets cultured in vitro. 2. More than 30% of explants from young leaflets of 2 to 3-day old seedlings regenerated shoots. But no morphogenesis was observed when leaflets from 5 or 10-day old seedlings were excised. 3. Plant regeneration can be done sequentially by inducing shoot and root formation. (author abst.) |
