Science Links Japan | Induction of Dog Sperm Capacitation by Glycosaminoglycans and Glycosaminoglycan Amounts of Oviductal and Uterine Fluids in Bitches.

Induction of Dog Sperm Capacitation by Glycosaminoglycans and Glycosaminoglycan Amounts of Oviductal and Uterine Fluids in Bitches.

Accession number;00A0211379

Title;Induction of Dog Sperm Capacitation by Glycosaminoglycans and Glycosaminoglycan Amounts of Oviductal and Uterine Fluids in Bitches.

Author;KAWAKAMI E(Nippon Veterinary And Animal Sci. Univ., Tokyo, Jpn) ARAI T(Nippon Veterinary And Animal Sci. Univ., Tokyo, Jpn) OISHI I(Nippon Veterinary And Animal Sci. Univ., Tokyo, Jpn) HORI T(Nippon Veterinary And Animal Sci. Univ., Tokyo, Jpn) TSUTSUI T(Nippon Veterinary And Animal Sci. Univ., Tokyo, Jpn)

Journal Title;J Vet Med Sci

Journal Code:F0905A

ISSN:0916-7250

VOL.62;NO.1;PAGE.65-68(2000)

Figure&Table&Reference;FIG.3, TBL.1, REF.38

Pub. Country;Japan

Language;English

Abstract;Ejaculated sperm collected from 12 beagle dogs were incubated in canine capacitation medium(CCM), supplemented with 5.MU.g/ml chondroitin sulfate A(CS), 5.MU.g/ml hyaluronic acid(HA), or 5.MU.g/ml heparin(HP) for 7hr at 38.DEG.C. in a 5% CO2 in air atmosphere to investigate the effects of glycosaminoglycans(GAGs) on dog sperm capacitation. The percentages of motile sperm, hyperactivated sperm(%HY), and acrosome-reacted sperm(%AR) in all media were examined after 4hr and 7hr of incubation. The oviducts and uteri of 9 anestrous and 18 estrous beagle bitches were removed under halothane inhalation anesthesia to measure the total GAG amounts in oviductal and uterine fluids. The lumens of the ampulla of the oviducts, isthmus of the oviducts, and the uterine horns were each flushed with 1ml HEPES-EDTA fluid. Total GAG amounts in the flush fluids obtained were measured with a spectrophotometer. Sperm motility(51-59%), %HY(79-86%), and %AR(31-36%) in CCM supplemented with CS,HA, or HP were significantly higher after 7hr of incubation than when incubated in CCM without GAGs(P<0.01 or 0.05). The mean total GAG amounts in the fluids from the ampulla and isthmus of the oviducts and the uterine horns in the estrous bitches were higher than in the anestrous bitches. These results indicate that GAGs in the oviductal and uterine fluids in estrous bitches are associated with in vivo sperm capacitation. (author abst.)