Science Links Japan | Changes in Diameter Size and F-Actin Expression in the Myocytes of Patients With Diabetes and Streptozotocin-Induced Diabetes Model Rats.

Changes in Diameter Size and F-Actin Expression in the Myocytes of Patients With Diabetes and Streptozotocin-Induced Diabetes Model Rats.

Accession number;00A0092352

Title;Changes in Diameter Size and F-Actin Expression in the Myocytes of Patients With Diabetes and Streptozotocin-Induced Diabetes Model Rats.

Author;KAWAGUCHI MICHIKO(Fukushima Med. Coll.) ASAKURA TSUKASA(Fukushima Med. Coll.) SAITO FUJIKO(Fukushima Med. Coll.) NEMOTO OSAMU(Fukushima Med. Coll.) MAEHARA KAZUHIRA(Fukushima Med. Coll.) MIYAKE KATSUYA(Fukushima Med. Coll.) SUGAI NAONORI(Fukushima Med. Coll.) MARUYAMA YUKIO(Fukushima Med. Coll.)

Journal Title;J Cardiol

Journal Code:Y0264A

ISSN:0914-5087

VOL.34;NO.6;PAGE.333-339(1999)

Figure&Table&Reference;FIG.3, TBL.2, REF.25

Pub. Country;Japan

Language;Japanese

Abstract;Diabetes mellitus may be an independent risk factor for disturbance of cardiac function, but the detailed mechanism remains unclear. In the present study, histological examinations were carried out on 25 hearts from diabetes model rats as well as myocardial biopsy materials from patients with diabetes (n=25). The mean diameter of the cardiac myocytes in humans was 12.2.+-.0.5.MU.m in the control group of patients without diabetes mellitus or hypertension (n=6), 13.7.+-.0.8.MU.m in the hypertension group (n=3), 9.0.+-.1.7.MU.m in the diabetes group (n=8), and 11.9.+-.2.0.MU.m in the diabetes with hyperension group (n=8). The cardiac myocytes of diabetic patients appeared to be atrophic. Comparison of the size of myocytes in the control rats vs streptozotocin-induced diabetes model rats (n=7, each) was 5.4.+-.0.2 vs 5.2.+-.0.3.MU.m at 2 weeks; 5.9.+-.0.1 vs 4.9.+-.0.9.MU.m at 12 weeks, and 5.7.+-.0.1 vs 4.0.+-.0.2.MU.m at 24 weeks, respectively, and gradually decreased in streptozotocin rats with aging. Immuno-histochemistry with phaloidin was used to assess F-actin in the cardiac myocytes. The relative cross-sectional area of F-actin in the cardiac myocytes of streptozotocin rats was compared to that in non-streptozotocin rat myocytes. F-actin fluorescence in streptozotocin rats was 89.9.+-.3.9% at 2 weeks, 77.9.+-.6.4% at 12 weeks, and 56.8.+-.5.7% at 24 weeks, indicating a decrease in F-actin. These results suggest that the smaller myocytes observed in patients with diabetes and streptozotocin rats are related to the decrease in F-actin in myocytes. (author abst.)