The cDNA Sequences of Equine Antioxidative Enzyme Genes Cu/Zn-SOD and Mn-SOD, and These Expressions in Equine Tissues.
| Accession number;99A0377872 |
| Title;The cDNA Sequences of Equine Antioxidative Enzyme Genes Cu/Zn-SOD and Mn-SOD, and These Expressions in Equine Tissues. |
| Author;ISHIDA N(Japan Racing Assoc., Tochigi, Jpn) KATAYAMA Y(Japan Racing Assoc., Tochigi, Jpn) SATO F(Japan Racing Assoc., Tochigi, Jpn) HASEGAWA T(Japan Racing Assoc., Tochigi, Jpn) MUKOYAMA H(Japan Racing Assoc., Tochigi, Jpn) |
Journal Title;J Vet Med Sci
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Journal Code:F0905A
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ISSN:0916-7250
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VOL.61;NO.3;PAGE.290-294(1999)
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| Figure&Table&Reference;FIG.4, REF.25 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The entire cDNA sequences were determined by reverse transcription-polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE) techniques for equine copper/zinc superoxide dismutase(Cu/Zn-SOD) and manganese superoxide dismutase(Mn-SOD) through the use of total RNA extracted from the testis of an adult Thoroughbred. The results revealed a protein coding region for equine Cu/Zn-SOD with bases totaling 465bp, accompanied by an estimated 154 residues of amino acids. As for equine Mn-SOD, its coding region contained a total of 669bp and an estimated 222 residues of amino acids. Further, the expression of Cu/Zn-SOD and Mn-SOD genes were confirmed in the equine tissues by RT-PCR and in situ hybridization. (author abst.) |
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