Changes in Transforming Growth Factor-.BETA. and Platelet-derived Growth Factor in the Optic Nerve Head in Monkey Experimental Glaucoma.

Accession number;99A0390123
Title;Changes in Transforming Growth Factor-.BETA. and Platelet-derived Growth Factor in the Optic Nerve Head in Monkey Experimental Glaucoma.
Author;FUKUCHI TAKEO(Niigata Univ., Sch. of Med.)   UEDA JUN(Niigata Univ., Sch. of Med.)   HANYU TAKAKO(Niigata Univ., Sch. of Med.)   ABE HARUKI(Niigata Univ., Sch. of Med.)   SAWAGUCHI SHOICHI(Univ. of Ryukyus, Fac. of Med.)   
Journal Title;Journal of Japanese Ophthalmological Society
Journal Code:Z0666A
ISSN:0029-0203
VOL.103;NO.3;PAGE.193-200(1999)
Figure&Table&Reference;FIG.5, REF.33
Pub. Country;Japan
Language;Japanese
Abstract;Purpose: Remodeling of the extracellular matrix occurs in the lamina cribrosa in progressed glaucomatous optic nerve damage including disc cupping. We examined immunohistochemical changes in the transforming growth factor(TGF)-.BETA. and platelet-derived growth factor(PDGF) in the optic nerve heads in experimentally induced glaucoma. Methods: We used 3 cynomolgus and 2 Japanese monkey eyes. Glaucoma was induced by repeated argon laser photocoagulation of the chamber angle. Eyes were enucleated after disc cupping had formed 3 to 5 months after treatment. The optic nerve head was examined for expression of TGF .BETA.1,.BETA.2, and .BETA.3, and PDGF A and B in frozen sections and by the biotin-ExtrAvidin-Alkali Phosphatase method. Findings Normal monkey eyes showed TGF .BETA.1,.BETA.2, and .BETA.3, and PDGF A, and B in the optic nerve head including the nerve fibers, glial cells, and vascular cells. Glaucomatous eyes showed stronger expression of TGF .BETA.1 and .BETA.2 in the glial cells around the lamina cribrosa. The staining intensities for TGF .BETA.3, PDGF A, and PDGF B were the same as in normal eyes. Conclusion: Eyes with experimental glaucoma showed higher expressions of TGF .BETA.1 and .BETA.2 around the lamina cribrosa. This finding may show upregulation of extracellular matrix production as related to remodeling of the lamina cribrosa in glaucoma. (author abst.)
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