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Accession number;99A0344213
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| Title;Determination of Vitamin E by HPLC. |
| Author;
TAKAHASHI TAKAHIRO
(Minist. of Financ., Cent. of Cust. Lab.)
IWASE KEN'ICHI
(Minist. of Financ., Cent. of Cust. Lab.)
KOGA SATOSHI
(Minist. of Financ., Cent. of Cust. Lab.)
SASAGAWA KUNIO
(Minist. of Financ., Cent. of Cust. Lab.)
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Journal Title;Reports of the Central Customs Laboratory
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Journal Code:S0329A
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ISSN:0286-1933
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VOL.;NO.38;PAGE.23-30(1998)
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| Figure&Table&Reference;FIG.6, TBL.3, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;A HPLC method for the determination of tocopherols in vegetable oil and its preparations was investigated. The GC method which is used as the analytical method for that in our laboratory has problems. Those are the complications of experimental procedure and no separation of .BETA.-and.GAMMA.-tocopherols.The HPLC separation was performed on a 4.6mm I.D*250mm SIL-5B column (column temperature 30.DEG.C.) with a mobile phase of n-Hexane: iso-Propyl alcohol (100:1) at a flow rate of 1.0 ml/ml. UV detection was performed with a wave length of 300 nm. It was found that the HPLC method is free from those problems and more useful than the GC method in our laboratory. (author abst.) |
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